My research investigates the role of noncanonical DNA structures, specifically guanine-rich G-quadruplexes (G4s), as therapeutic targets in cancer. This work combines biophysical, biochemical, and cellular methods in both 2D and 3D cancer models.
The approach has two main focuses: sensing and therapeutics. In therapeutics, small-molecule G4-stabilizing ligands and light-activated compounds are designed to selectively bind and stabilize G4s, inhibiting DNA replication or RNA transcription and promoting cancer cell death at low doses, with minimal off-target effects.
In sensing, supramolecular dyes with disaggregation-induced emission properties signal G4 structures, allowing real-time visualization of G4 dynamics in live cells. When combined with photosensitizers, these dyes act as theranostics, providing both diagnostic and therapeutic functions.
Additionally, the research explores the impact of hypoxia—a common condition in aggressive tumors—on G4 distribution. This aims to improve targeted therapies by enhancing our understanding of G4 stability and localization under low-oxygen conditions.